Comparative genomics reveals the in planta-secreted Verticillium dahliae Av2 effector protein recognized in tomato plants that carry the V2 resistance locus.
Identifieur interne : 000195 ( Main/Exploration ); précédent : 000194; suivant : 000196Comparative genomics reveals the in planta-secreted Verticillium dahliae Av2 effector protein recognized in tomato plants that carry the V2 resistance locus.
Auteurs : Edgar A. Chavarro-Carrero [Pays-Bas] ; Jasper P. Vermeulen [Pays-Bas] ; David E Torres [Pays-Bas] ; Toshiyuki Usami [Japon] ; Henk J. Schouten [Pays-Bas] ; Yuling Bai [Pays-Bas] ; Michael F. Seidl [Pays-Bas] ; Bart P H J. Thomma [Pays-Bas, Allemagne]Source :
- Environmental microbiology [ 1462-2920 ] ; 2020.
Abstract
Plant pathogens secrete effector molecules during host invasion to promote colonization. However, some of these effectors become recognized by host receptors to mount a defence response and establish immunity. Recently, a novel resistance was identified in wild tomato, mediated by the single dominant V2 locus, to control strains of the soil-borne vascular wilt fungus Verticillium dahliae that belong to race 2. With comparative genomics of race 2 strains and resistance-breaking race 3 strains, we identified the avirulence effector that activates V2 resistance, termed Av2. We identified 277 kb of race 2-specific sequence comprising only two genes encoding predicted secreted proteins that are expressed during tomato colonization. Subsequent functional analysis based on genetic complementation into race 3 isolates and targeted deletion from the race 1 isolate JR2 and race 2 isolate TO22 confirmed that one of the two candidates encodes the avirulence effector Av2 that is recognized in V2 tomato plants. Two Av2 allelic variants were identified that encode Av2 variants that differ by a single acid. Thus far, a role in virulence could not be demonstrated for either of the two variants.
DOI: 10.1111/1462-2920.15288
PubMed: 33078534
Affiliations:
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Chavarro-Carrero</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB</wicri:regionArea><wicri:noRegion>6708 PB</wicri:noRegion></affiliation></author><author><name sortKey="Vermeulen, Jasper P" sort="Vermeulen, Jasper P" uniqKey="Vermeulen J" first="Jasper P" last="Vermeulen">Jasper P. 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Schouten</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB</wicri:regionArea><wicri:noRegion>6708 PB</wicri:noRegion></affiliation></author><author><name sortKey="Bai, Yuling" sort="Bai, Yuling" uniqKey="Bai Y" first="Yuling" last="Bai">Yuling Bai</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB</wicri:regionArea><wicri:noRegion>6708 PB</wicri:noRegion></affiliation></author><author><name sortKey="Seidl, Michael F" sort="Seidl, Michael F" uniqKey="Seidl M" first="Michael F" last="Seidl">Michael F. Seidl</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB</wicri:regionArea><wicri:noRegion>6708 PB</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Theoretical Biology and Bioinformatics Group, Department of Biology, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Theoretical Biology and Bioinformatics Group, Department of Biology, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB</wicri:regionArea><wicri:noRegion>6708 PB</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Botanical Institute, Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Botanical Institute, Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author></analytic><series><title level="j">Environmental microbiology</title><idno type="eISSN">1462-2920</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant pathogens secrete effector molecules during host invasion to promote colonization. However, some of these effectors become recognized by host receptors to mount a defence response and establish immunity. Recently, a novel resistance was identified in wild tomato, mediated by the single dominant V2 locus, to control strains of the soil-borne vascular wilt fungus Verticillium dahliae that belong to race 2. With comparative genomics of race 2 strains and resistance-breaking race 3 strains, we identified the avirulence effector that activates V2 resistance, termed Av2. We identified 277 kb of race 2-specific sequence comprising only two genes encoding predicted secreted proteins that are expressed during tomato colonization. Subsequent functional analysis based on genetic complementation into race 3 isolates and targeted deletion from the race 1 isolate JR2 and race 2 isolate TO22 confirmed that one of the two candidates encodes the avirulence effector Av2 that is recognized in V2 tomato plants. Two Av2 allelic variants were identified that encode Av2 variants that differ by a single acid. Thus far, a role in virulence could not be demonstrated for either of the two variants.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33078534</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-2920</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>19</Day></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Comparative genomics reveals the in planta-secreted Verticillium dahliae Av2 effector protein recognized in tomato plants that carry the V2 resistance locus.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/1462-2920.15288</ELocationID><Abstract><AbstractText>Plant pathogens secrete effector molecules during host invasion to promote colonization. However, some of these effectors become recognized by host receptors to mount a defence response and establish immunity. Recently, a novel resistance was identified in wild tomato, mediated by the single dominant V2 locus, to control strains of the soil-borne vascular wilt fungus Verticillium dahliae that belong to race 2. With comparative genomics of race 2 strains and resistance-breaking race 3 strains, we identified the avirulence effector that activates V2 resistance, termed Av2. We identified 277 kb of race 2-specific sequence comprising only two genes encoding predicted secreted proteins that are expressed during tomato colonization. Subsequent functional analysis based on genetic complementation into race 3 isolates and targeted deletion from the race 1 isolate JR2 and race 2 isolate TO22 confirmed that one of the two candidates encodes the avirulence effector Av2 that is recognized in V2 tomato plants. Two Av2 allelic variants were identified that encode Av2 variants that differ by a single acid. Thus far, a role in virulence could not be demonstrated for either of the two variants.</AbstractText><CopyrightInformation>© 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Chavarro-Carrero</LastName><ForeName>Edgar A</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Vermeulen</LastName><ForeName>Jasper P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>E Torres</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Theoretical Biology and Bioinformatics Group, Department of Biology, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Usami</LastName><ForeName>Toshiyuki</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Graduate School of Horticulture, Chiba University, Matsudo, Chiba, 271-8510, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schouten</LastName><ForeName>Henk J</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bai</LastName><ForeName>Yuling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Seidl</LastName><ForeName>Michael F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Theoretical Biology and Bioinformatics Group, Department of Biology, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Thomma</LastName><ForeName>Bart P H J</ForeName><Initials>BPHJ</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-4125-4181</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, 6708 PB, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Botanical Institute, Cologne, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Consejo Nacional de Ciencia y Tecnología</Agency><Country></Country></Grant><Grant><GrantID>390686111</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country></Country></Grant><Grant><Agency>Foundation Topconsortium voor Kennis en Innovatie</Agency><Country></Country></Grant><Grant><Agency>Nederlandse Organisatie voor Wetenschappelijk Onderzoek</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>29</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33078534</ArticleId><ArticleId IdType="doi">10.1111/1462-2920.15288</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Alexander, L.J. 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Thomma</name><name sortKey="Vermeulen, Jasper P" sort="Vermeulen, Jasper P" uniqKey="Vermeulen J" first="Jasper P" last="Vermeulen">Jasper P. Vermeulen</name><name sortKey="Vermeulen, Jasper P" sort="Vermeulen, Jasper P" uniqKey="Vermeulen J" first="Jasper P" last="Vermeulen">Jasper P. Vermeulen</name></country><country name="Japon"><noRegion><name sortKey="Usami, Toshiyuki" sort="Usami, Toshiyuki" uniqKey="Usami T" first="Toshiyuki" last="Usami">Toshiyuki Usami</name></noRegion></country><country name="Allemagne"><noRegion><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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